FluoroCet Exosome Quantitation Kit
| Specifications | |
|---|---|
| Product Category: | FLUOROCET Exosome Quantitation |
Product Description
- Sensitive: Requires as little as 500 ng of exosomal protein
- Perfect for clinical samples or any situation where samples are limiting (e.g. low volume tissue culture or model organisms with limited biofluid availaibility)
- Fast—complete assay based on AChE activity in exosomes: only sixty minutes
- Versatile—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoffinity capture)
- Quantitative—internal assay standards enable estimation of exosome abundance
Enabling highly sensitive quantitation of exosomes in only 60 min, the FLUOROCET™ kit (Cat# FCET96A-1-SBI) takes SBI's popular EXOCET assay (see Product Line Overview) and increases the sensitivity over 30-fold (see data below).
Fully compatible with all of SBI’s ExoQuick™ exosome isolation reagents as well as ultracentrifugation, FLUOROCET is great for quantifying exosomes when samples are limiting. Like EXOCET, FluoroCet measures the activity of acetecylcholinesterase (AChE), a known exosome protein, whilst the more sensitive FLUOROCET assay relies on fluorescence at 495 nm. To enable estimation of exosome abundance in your target sample, each kit includes a standard curve calibrated to known amounts of human exosomes as determined by NanoSight analysis. The FLUOROCET kit contains all of the reagents needed to perform 96 reactions.
How It Works
Exosome quantitation with FluoroCet is quick and easy
- Calibrate your fluorescence plate reader
- Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
- Lyse exosomes with the included Exosome Lysis Buffer
- Measure AChE activity—add the included buffer and incubate for 20 minutes (or appropriate time as determined during the calibration step) at room temperature in the dark, and then measure fluorescence using excitation at 530–570 nm and emission at 590–600 nm.
Supporting Data
Fig.1. Comparison of limit of detection (LOD) of exosomal protein by EXOCET versus FLUOROCet. Data from FLUOROCet remains linear—and quantitative—even at low exosome amounts.
- Catalog Number
FCET96A-1-SBI - Supplier
SBI System Biosciences - Size
- Shipping
Blue Ice
