ExoQuick-TC Exosome Precipitation Solution

Product Description

• Saves time and labor
• Is easily scalable
• Conserves precious sample
• Delivers high yields of functional, high quality exosomes
• Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, basic biology, such as role in tumorigenesis.

ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media, and from certain biofluids such as saliva, urine, follicular fluid, and breast milk. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.

How it works

ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

To isolate exosomes from tissue culture media, simply:

1. Add an appropriate volume of ExoQuick-TC
2. Incubate overnight at 4°C
3. Isolate exosomes with a 30-minute low-speed spin (1500g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

Performance Data

Fig.1. Exosome size distribution and yields from tissue culture media. Cells from a human HT1080 lung sarcoma cell line were cultured in conditioned media (serum-free) for 72 hours. 10 mL of media was combined with 2 mL ExoQuick-TC, incubated overnight at 4°C, and centrifuged at 1500g for 30 minutes to isolate exosomes. The exosome pellet was resuspended in 1 mL PBS, diluted 1:40, and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick-TC recovered 133 nm exosomes at a concentration of 1.74 x 10⁹ particles/mL. 

More data of performance can be found in the ExoQuick-TC Product Sheet.

Choose the right ExoQuick for your application