ExoQuick-TC PLUS Exosome Purification Kit - for tissue culture media

Product Description

ExoQuick-TC PLUS - Quick and Efficient Exosome Isolation for Sensitive Applications, when you need an extra level of confidence

• Better—delivers as much as 150-fold higher yields than ultracentrifugation-based methods
• Cleaner—dramatically reduces the amount of carry-over protein compared to other column- or precipitation-based kits, and even ultracentrifugation-based methods
• Powerful—detects biomarkers with as much as a 3-fold increase in sensitivity
• Fast—takes less than 10 minutes of hands-on-time after initial ExoQuick isolation

While the ExoQuick® family of reagents speed-up and simplify exosome isolation and are compatible with most downstream uses, some applications are especially sensitive to carry-over of non-exosomal proteins applications such as mass spectrometry, western blotting of low abundance markers, exosome labeling, and in vivo/ex vivo exosome delivery. Which is why SBI offers ExoQuick PLUS and ExoQuick-TC PLUS.

ExoQuick-TC PLUS can be used to purify exosomes from a wide variety of tissue culture media, and from certain biofluids such as saliva, urine, follicular fluid, and breast milk With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC PLUS is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples that will then be used in sensitive downstream applications.

How it Works

ExoQuick-TC PLUS further reduces protein carryover from serum/plasma and tissue culture exosomal preparations with a simple treatment by ready-to-use, customized microspheres (kits contains enough ExoQuick and pre-packed microspheres for 10 reactions. In less than 10 minutes of total hands-on time after ExoQuick isolation, you can obtain highly pure, intact exosomes suitable for a variety of protein-sensitive applications.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

1. Add an appropriate volume of ExoQuick-TC PLUS to as little as 100 µl sample
2. Incubate for at least one hour at 4°C
3. Pellet exosomes with a 30-minute low-speed spin (1500g)
4. Resuspend pellet, add ExoQuick PLUS Microspheres, and incubate for 10 minutes at 4°C
5. Remove microspheres with a 30-minute low-speed spin (1500g)

Exosome Isolation with ExoQuick-TC PLUS saves time and results in higher protein yields and less albumin carryover compared to Ultracentrifugation:

Fig.1. Workflow of ExoQuick PLUS compared to Ultracentrifugation.

Performance Data

ExoQuick-TC PLUS provides efficient exosome isolation with reduced protein carry-over, resulting in isolated exosomes suitable for most sensitive downstream applications.

Fig.2. Comparison of residual protein carry-over from bovine-exosome depleted media (Exo-FBS) treated with ExoQuick-TC PLUS or Company CGS’s product. ExoQuick-TC PLUS treatment showed significantly lower protein carry-over from the bovine-exosome depleted media. For each lane, 5 µg of total protein was loaded. After running the gel, total protein was detected using colloidal blue staining. Data courtesy of Richard J. Jones, PhD., MD Anderson Cancer Center.

Fig.3. Exosome isolation with ExoQuick-TC PLUS results in cleaner preparations. ZetaView particle size analysis of exosomes isolated from cultured RPMI 8226 cells using either ExoQuick-TC PLUS (left panel) or a competitor’s kit (right panel). The red double arrow in the left panel indicates a broad shoulder of particles too small to be exosomes (< 50 nm in diameter) which are likely to be undesirable protein aggregates. These contaminants are not present in the exosomes isolated using ExoQuick-TC PLUS (red double arrow indicates the same particle diameter range as in the right panel). Data courtesy of Richard J. Jones, PhD., MD Anderson Cancer Center.

More data of performance can be found in the ExoQuick PLUS Product Sheet.

Choose the best ExoQuick product for your application.