ExoQuick-CG cGMP-grade Exosome Isolation reagent (50 ml)

Product Description

• 21 CFR 820 compliant
• All ingredients sourced from GMP manufacturer
• Reagent mixed under cGMP conditions at GMP facility
• Triple filtered
• Sterility tested
• Works in all biofluids tested

ExoQuick-CG enables cGMP quality exosome isolation for pre-clinical applications. High-throughput, quantitative isolation of exosomes from most biofluids are possible. Compatible with a wide variety of downstream applications, ExoQuick-CG is an effective and proven alternative to ultracentrifugation. Based on SBI’s well-regarded ExoQuick-TC, SBI's ExoQuick-CG exosome precipitation reagent makes miRNA and protein biomarker discoveries simple, reliable, and quantitative. Enrich for exosomal miRNAs with ExoQuick-CG and accurately profile them using SBI's SeraMir qPCR arrays. Downstream protein analysis is also possible with SBI's exosome specific antibodies and ELISA kits.

How it works

ExoQuick-TC can be used to purify exosomes from a wide variety of  tissue culture media, and from certain biofluids such as saliva, urine, follicular fluid, and breast milk. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.

To isolate exosomes from tissue culture media, simply:

• Add an appropriate volume of ExoQuick-CG
• Incubate for at least one hour at 4°C
• Isolate exosomes with a 30-minute low-speed spin (1500g).

Fig.1. Isolated exosomes can be found in the pellet and resuspended in an appropriate solution. 

Performance Data

ExoQuick-CG Supports Exosome Isolation from a Wide Range of Biofluids

With ExoQuick-CG, you can quickly and easily isolate high yields of exosomes using cGMP-grade reagents for any downstream application.

Exosome Size Distribution and Yields Using ExoQuick-CG

NanoSight analysis shows ExoQuick-CG isolates high concentrations of exosomes from a variety of biofluids, and that the isolated exosomes are in the expected size range.

Figure 2. 3.3 mL of ExoQuick-CG was combined with 10 mL of conditioned media from either Human HT1080 lung sarcoma cells or Mouse JAWS II dendritic cells that had been cultured in Exo-FBS (exosome depleted FBS medium supplement, catalog # EXO-FBS-50A-1). All samples were incubated overnight at 4°C for exosome precipitation. Exosomes were resuspended in 0.5 mL of PBS and visualized on a NanoSight LM10 instrument.

Exosome CD63 Western Blot Validation Studies

Western blotting of the ExoQuick-CG-isolated particles using an exosome-specific antibody (anti-CD63) further validates that the particles isolated with ExoQuick-CG are exosomes.

Figure 3. Approximately 10-50 µg of exosomes from the NanoSight studies shown in Figure 1 were separated via denaturing SDS PAGE for Western blot analysis. CD63 protein was detected using SBI’s rabbit anti-CD63 primary antibody and SBI’s HRP-conjugated secondary goat anti-rabbit antibody (catalog # EXOAB-CD63A-1).

Choose the right ExoQuick for your application