ExoQuick Exosome Precipitation Solution

Product Description

• Saves time and labor
• Is easily scalable
• Conserves precious sample
• Delivers high yields of functional, high quality exosomes
• Can be used to isolate exosomes for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, such as in cell-to-cell signaling, basic biology, such as role in tumorigenesis.

ExoQuick can be used to purify exosomes from plasma, serum, and malignant ascites. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.

To isolate exosomes from plasma, we recommend pre-treatment with the Thrombin Plasma Prep or using the ExoQuick™ Plasma Prep and Exosome Precipitation Kit. 

How it works

ExoQuick is a proprietary polymer that gently precipitates exosomes. Add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

To isolate exosomes from cleared serum, plasma, or ascites fluid, simply:

1. Add an appropriate volume of ExoQuick to as little as 100 µl sample
2. Incubate for at least one hour at 4°C
3. Isolate exosomes with a 30-minute low-speed spin (1500 g).

Fig.1. Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

Performance Data

Fig. 2. Exosome precipitation from 250 ul human serum samples was verified by Western blot using antibodies against two commonly found exosome biomarker proteins, CD63 and CD9 and small RNAs analyzed on RNA gels after Trizol extraction.

More data of performance can be found in the ExoQuick Product Sheet.

Choose the right ExoQuick for your application