CUTANA CUT&Tag Kit Version 2, Primer Set 2

Ultra Low Input Chromatin Mapping Assay

Product Description

• Fast: Cells to sequencing in 2 days
• Streamlined: Exclusive single-tube protocol, no library prep
• High Sensitivity: Reliable data down to 10,000 cells
• Scalable: Designed for multi-channel pipetting and 8-strip tubes
• Dramatic cost savings: Only 5-8 million sequencing reads

Cleavage Under Targets and Tagmentation (CUT&Tag) uses a fusion of protein A, protein G, and Tn5 (pAG-Tn5) to selectively target antibody-labelled chromatin for high-resolution sequencing analysis. EpiCyphers CUTANA CUT&Tag Kit enables the use of this innovative mapping assay.

The CUTANA CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). This kit uses an exclusive Direct-to-PCR strategy to go from cells to PCR-amplified sequencing libraries in one tube, bypassing traditional library prep and minimizing sample loss. The protocol is also designed for compatibility with multi-channel pipetting for increased throughput and reproducibility. 

The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs. For chromatin-associated proteins (e.g. transcription factors), CUT&RUN is recommended.

Multiplexed Primers

The CUTANA Cut & Tag Kit is available in 2 versions. Each kit contains combinatorial dual indices for multiplexed sequencing of up to 48 reactions. Customers can combine the kits to multiplex up to 96 reactions:

CUTANA CUT&Tag Kit Version 2, Primer Set 1
CUTANA CUT&Tag Kit Version 2, Primer Set 2

Included Reagents

• pAG-Tn5 for targeted chromatin tagmentation
• ConA magnetic beads for immobilizing cells/nuclei
• SNAP-CUTANA™ Spike-in Controls for assay optimization and reaction monitoring
• Positive (H3K27me3) and negative (IgG)
• Anti-Rabbit Secondary Antibody 
• i5 and i7 primers, PCR Master Mix, and SPRI beads for indexing PCR and library purification
• 8-strip tubes, buffers, buffer components
  
  

Learn more about the CUT&Tag Technology

Supported Applications

• Single Cell CUT&Tag
• Spatial CUT&Tag
• Multiomic CUT&Tag

Related Products

Individual core reagents and antibodies for CUTANA CUT&Tag Assays are also available.