Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag Workflows

Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope.

Product Description

Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope. 

For best results, this antibody should be used with CUTANA™ pAG-Tn5 and CUTANA Compatible Antibodies.

Performance Data

Figure 1: CUT&Tag data
CUT&Tag was performed on 100k K562 nuclei with rabbit anti-H3K27me3 antibody (EpiCypher) and 0.5 µg of anti-rabbit secondary antibody for CUTANA™ CUT&Tag using the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag protocol. Gene browser shots generated using the Integrative Genomics Viewer (IGV, Broad Institute) show a representative 324 kb window centered at the LAMC3 gene. The genomic distribution pattern was consistent with that of H3K27me3.