plexWell™ LP384 (low-pass whole genome) multiplexed Library Prep Kit with ANY INDEX SET

Product Description

plexWell libraries are conveniently prepared in a single tube after a single tagging step, which reduces associated labor, consumable and library QC costs by more than 10-fold.

• plexWell™ NGS multiplexed library generation kit for Illumina®
• Assay-ready 96-well fully-skirted low-profile PCR plates (4 x 96)
• 2,304 (96 x 24) unique barcode combinations:
  6 sets of 4 pool barcodes available – PB Set A, B, C, D, E, or F
• With all major reagents necessary to prepare libraries including Magwise™ paramagnetic beads (DNA polymerase not included)
• Normalizes input DNA over wide input range of 5-25 ng
  
  

plexWell Low-pass 384 Library Preparation Kits come in an assay-ready 96-well configuration to streamline high-throughput multiplexed library preparation. Each kit contains sufficient reagents to prepare dual-indexed Illumina-compatible libraries from 384 individual DNA samples. The kit comes in versions A, B, C, D, E, and F allowing users to multi-plex up to 2304 samples, if required. Multiple DNA types are suitable input for the kit, ranging in size and complexity for up to 20 million read pairs per sample. plexWell libraries are compatible with the Illumina iSeq, MiniSeq MiSeq, NextSeq, HiSeq and NovaSeq systems.

plexWell library preparation kits from seqWell utilize proprietary transposase-based reagents to insert barcoded adapters directly into input DNA in two separate steps.

plexWell Library Prep Workflow

plexWell Library Prep Chemistry

Recommended Applications

• Low-depth whole genome/GBS coupled with an imputation and analysis software

Specifications

Time-to-prepare a multiplexed library from 96 samples:

• Hands-on time:  50 min
• Total elapsed time:  3 hrs

Recommended input:

• 5-25 ng of genomic DNA per sample (n=96)

Expected Results:

• Library Yield: 750 – 1500 fmoles of purified multiplexed library
• Estimated Duplication Rate: 10% per 10M paired-reads on human gDNA
• Substantial normalization: Typically <2.5-fold range (min to max) in read count across all plexWell pools

Compatibility with Illumina® sequencing systems:

• Compatible with Illumina MiSeq®, NextSeq®, HiSeq® and NovaSeq® instrument platforms

Additional Resources

plexWell Kits Master Index List (download)

plexWell LP 384 Library Preparation Kit: Cost-effective High-throughput Low Pass Genotyping (poster)

plexWell™ Low Pass 384 Library Preparation Kit for Microbiome Applications (poster)