DepleteX RNA Depletion (Globin)

Focused RNA depletion panel for increasing detection sensitivity of low expressing transcripts in blood derived samples

Product Description

DepleteX globin RNA depletion panel removes four highly expressed globin transcripts in-vitro ahead of sequencing, redistributing sequencing clusters to unique biologically relevant transcripts —allowing you to maximize UMI and gene sensitivity.

Beyond ribodepletion, RNA samples typically have a small number of highly expressed, well characterized transcripts, and resequencing these transcripts adds little biologically relevant information. Unfortunately, these highly expressed transcripts outcompete low expressing transcripts for sequencing coverage. This lack of sequencing coverage coupled with significant noise from high expressing transcripts limits detection of low expressing genes, rare variants and unique fusion events that may be disease drivers.

• Globin panel designed to deplete overexpressing transcripts in blood derived samples
• Re-assign sequencing reads to lower expressing transcripts for increased coverage​
• Maximize variant and isoform detection with less sequencing
• Designed to deplete 4 globin genes

Content

The kit contains globin-specific guide RNA and depletion reagents for post-library depletion.

Compatible Kits

CRISPRclean Stranded Total RNA Prep Kit with rRNA Depletion
DepleteX Single Cell RNA Boost Kit