DepleteX RNA Depletion Panel (Liver)

Pinpoint low expressing transcripts with our focused RNA depletion panel for liver tissue

Product Description

RNA samples typically have a small number of highly expressed, well-characterized transcripts that outcompete low expressing transcripts for sequencing coverage. Resequencing these transcripts adds little biologically relevant information. The lack of sequencing coverage, coupled with significant noise from high expressing transcripts, limits detection of low expressing genes, rare variants and unique fusion events that may be disease drivers.

CRISPRclean leverages Cas9 depletion, and a guide set designed to remove ~50% of the highest expressing transcripts from tissues, as determined by queries from the GTEx portal. These guide sets were designed to maximize the re-assignment of sequencing reads by removing a minimal number of gene encoding transcripts—preserving relevant and novel biological transcripts. These panels were designed for RNA-Seq libraries that are already treated with ribodepletion or poly-A selection.

• Liver panel designed to deplete ~50% of expressed transcripts in a normal liver tissue​
• Increase sensitivity by ~50% to see more biologically relevant transcript
• Maximize variant and isoform detection with less sequencing
• Integrate easily into your workflow with a simple and flexible protocol

Content

The kit contains tissue-specific guide RNA and depletion reagents for post-library depletion.

Compatible Kits

CRISPRclean Stranded Total RNA Prep Kit with rRNA Depletion
DepleteX Single Cell RNA Boost Kit