Immobilization Buffer Optimization Kit

Product Description

Immobilization buffer is used in label-free surface plasmon resonance instruments to enable pre-concentration of the ligand to be immobilized. Using an appropriate immobilization buffer will result in much higher ligand density on the surface of the sensor. The optimal pH is dependent on the pI of the ligand and should be determined experimentally if the pI is not known. In an immobilization experiment, the ligand is diluted in immobilization buffers with pH values that differ by half a unit. The pH which gives the highest immobilization levels with the highest pH is the optimal buffer to use. Immobilization buffers should also have a low ionic strength.

Nicoya's 10mM Acetate Buffer is an excellent immobilization buffer for many applications where the ligand pI is below 6. It is available in a range of pH values and as an optimization kit to help you determine the optimal conditions for you experiment. They come in 50mL and are degassed, ready for use.