Tagify™ i5 UMI Adapter-loaded Transposase Reagent Set

Product Description

Tagmentation-based NGS library preparation with simple, scalable, and reliable unique molecular identifier (UMI) addition can be achieved with this Tagify™ i5 UMI Adapter-loaded Transposase reagents. Accelerate your variant calling efforts with peace of mind.

• Quickly and reliably fragment and tag genomic DNA via loaded Tn5 transposase
• Sequence tag includes Illumina-compatible i5 barcode and UMIs
• Fully QC’d loaded transposase for reproducible tagmentation with certificate of analysis available

Applications* include targeted sequencing assays, counting assays requiring UMI addition, CRISPR QC, and cell and gene editing QC.

Tagify i5 UMI Adapter-loaded Transposase

• Each transposase is loaded with a full-length Illumina-compatible P5 + i5 + UMI + R1 adapter
• 10-bp i5 barcode and 10-bp UMI
• Available in sets of 24 or 96 loaded transposases

Tagify UMI in Action

Tagmenting DNA using Tagify i5 UMI loaded transposases requires a single 15-minute incubation at 55°C followed by reaction clean-up.  Tagged DNA can then seamlessly integrate into downstream applications* such as gene editing assays like UDiTaS, RGEN, TTIS-seq, CHANGE-seq and others. 

Applications*
Commercial use may require a license from a third party.
1. UDiTaS Method: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861650/
2. RGEN-Seq Method: https://pubmed.ncbi.nlm.nih.gov/34880355/

Additional Resources

Multiplexed Transposase-Based NGS Tools for QC Assays in Genome Editing Applications (Poster)
Tagify™ Transposase Reagent and Input DNA Titration (Technical Note)