ExpressPlex 2.0 Library Prep Kit, 384-well format

Featuring high-performance transposase TnX for reduced bias and improved coverage uniformity

Product Description

ExpressPlex 2.0 Early Access: The workflow simplicity of one-step library prep combined with the performance of TnX for high throughput sequencing of plasmids, amplicons and other synthetic constructs.

Improved performance: TnX transposase for reduced bias and improved coverage uniformity
Ease of use: Fragmentation, barcoding, and amplification in a single step
Rapid: 100-minute workflow (30 minutes or less hands-on) with pre-plated, reaction-ready reagents
Scalable: High-level sample multiplexing (up to 6,144 total barcode combinations) and auto normalization
ExpressPlex 2.0 Kits are available in 96- and 384-well format and with different Index-Sets

The patent-pending ExpressPlex 2.0 Library Prep Kit comes in a convenient 384-well PCR plate configuration for high-throughput multiplexed library preparation. This upgraded version of ExpressPlex uses seqWell’s high performance TnX™ transposase that was specially engineered for NGS library preparation. Amplicons (>350 bp) and plasmid DNA are suitable standard inputs for the kits. Appendix E highlights modifications that can be made for small microbial whole genome sequencing. ExpressPlex libraries are compatible with the Illumina MiSeq™, NextSeq™, iSeq™, and NovaSeq™ sequencing platforms.

Each ExpressPlex 2.0 – 384-well kit contains sufficient reagents to prepare Illumina-compatible libraries from 384 or 1,536 individual DNA samples. Libraries are standardly prepared with 384 samples per pool, up to 1,536 samples per kit. There are four different kits available for preparing libraries from 1,536 samples, providing a total of 6,144 total barcode combinations that can be loaded on a single sequencing run.

ExpressPlex Library Preparation Workflow

Expressplex 384 Wfg

Kit Content

Indexing Reaction Plate(s)
Ready Reaction Mix Plate(s)
MAGwise Paramagnetic Beads*

*A larger 15ml bottle of MAGWise paramagnetic beads is available (REF 101002) for purchase if desired for easier automation.

Supporting Data

TnX Transposase: Quality Libraries for Quality Sequencing

ExpressPlex 2.0 uses tagmentation powered by TnX, the next generation transposase, to simultaneously fragment & index sample DNA. Average fragment size ranges from 400-1200 bp.

Fig.2. Typical fragment profile following analysis on an Agilent TapeStation System. Data shown are for a 96-plex sample.

TnX: Transposase Engineered for Reduced Insertion Bias

ExpressPlex 2.0 is the only library prep kit powered by TnX! TnX was engineered to reduce insertional bias to improve uniformity of coverage. When directly compared to a Tn5-based competitor library prep kit for sequencing of 2 different plasmids, ExpressPlex 2.0 produced significantly lower CVs and range of coverage (max/min).

Tnx Reduced Bias

Fig.3. Library preparation for pUC19 and pMal-c67 was conducted using ExpressPlex 2.0 kit and a Tn5-based competitor kit using standard manufacturers’ protocols. Libraries were sequenced using an Illumina MiSeq.