CRISPR/Cas9 Genome Engineering

All you need for CRISPR/Cas9-based genome engineering

The recent discovery of the type II prokaryotic CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, originally discovered in the bacterium Streptococcus pyogenes that works as a mechanism to defend against viruses and foreign DNA, has provided a revolutionary tool for targeted genome engineering with simple elegance.

The system uses a nuclease, CRISPR-associated protein 9 (Cas9), that complexes with small guide RNAs (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adjacent motif (PAM) in any genomic location.

BioCat offers a comprehensive portfolio for CRISPR/Cas9-based genome engineering including vector-based and Cas9 protein/gRNA-based systems, see below. For screening projects lentiviral gRNA libraries and Cas9 expressing cell lines are provided.

• CRISPR/Cas9 Lentiviral sgRNA Libraries
• CRISPR/Cas9 Gene Knockout Kits with Donor Template
• CRISPR/Cas9 Gene Knockout Constructs
• CRISPRa Gene Activation Constructs
• CRISPR sgRNA Cloning & Control Vectors
• Cas9 and dCas9 Expression Constructs
• CRISPRa and CRISPRi Cloning & Control Vectors
• CRISPR/Cas9 Safe Harbor Transgene Knockin System
• Cas9 Expressing Cell Lines
• dCas9 Expressing Cell Lines
• CRISPR Knockout Cell Lines
• Cas9 and dCas9 Activity Assays
• Cas9 ELISA Kits
• CRISPR-mediated Gene Editing Detection

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